Magni:Back Door: Difference between revisions

Revision as of 10:40, 28 March 2013

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Ampicillin - 100 mg/ml in deionized water (-20°C)
Chloramphenicol - 34 mg/ml in ethanol (-20°C)
Kanamycin - 50 mg/ml in deionized water (-20°C)
Gentamycin - 50 mg/ml in deionized water (-20°C)
G418 - 50 mg/ml in deionized water (+4°C)
HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
IPTG Ready Made - 200 mM (-20°C)
X-Gal - 40 mg/ml in DMF (-20°C)

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-== General ==
+== Antibiotics, inducers and substrates ==
-http://www.labmedinfo.org/
+*Ampicillin - 100 mg/ml in deionized water (-20°C)
+*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
-http://cit.unipv.it/cit/
+*Kanamycin - 50 mg/ml in deionized water (-20°C)
+*Gentamycin - 50 mg/ml in deionized water (-20°C)
-== Reagents ==
+*G418 - 50 mg/ml in deionized water (+4°C)
+*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
+*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
+*IPTG Ready Made - 200 mM (-20°C)
+*X-Gal - 40 mg/ml in DMF (-20°C)
 == ''E. coli'' strains ==
+*BW20767 (Registry)
-== Important glycerol stocks ==
+*BW23474 (CGSC)
+*BW25141 (CGSC)
+*BW25142 (CGSC)
+*CW2553 + pJat8 (Registry)
+*DB3.1 (Invitrogen)
+*DH5alpha (Invitrogen)
+*KRX (Promega)
+*MC1061 (CGSC)
+*MG1655 (CGSC)
+*MG1655-Z1 (Univ of Washington)
+*TOP10 (Invitrogen)
+*W3110 (DSMZ)
+*XL1-Blue (Stratagene)
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