Magni:Back Door

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Current revision (12:40, 28 March 2013) (view source)
(E. coli strains)
 
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== Antibiotics ==
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== Antibiotics, inducers and substrates ==
*Ampicillin - 100 mg/ml in deionized water (-20°C)
*Ampicillin - 100 mg/ml in deionized water (-20°C)
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
*Kanamycin - 50 mg/ml in deionized water (-20°C)
*Kanamycin - 50 mg/ml in deionized water (-20°C)
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*Gentamycin - 50 mg/ml in deionized water (-20°C)
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== Inducers and substrates ==
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*G418 - 50 mg/ml in deionized water (+4°C)
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*HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C)
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*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
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*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol 70% or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
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*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
*IPTG Ready Made - 200 mM (-20°C)
*IPTG Ready Made - 200 mM (-20°C)
*X-Gal - 40 mg/ml in DMF (-20°C)
*X-Gal - 40 mg/ml in DMF (-20°C)
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== ''E. coli'' strains ==
== ''E. coli'' strains ==
*BW20767 (Registry)
*BW20767 (Registry)
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*BW23474 (CGSC)
*BW23474 (CGSC)
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**pir-116 strain, used to propagate the R6K conditional replication origin at high copy
 
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*BW25141 (CGSC)
*BW25141 (CGSC)
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**pir+ strain, used to propagate the R6K conditional replication origin at medium copy
 
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*BW25142 (CGSC)
*BW25142 (CGSC)
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**pir-116 strain, used to propagate the R6K conditional replication origin at high copy (equivalent to BW23474)
 
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*CW2553 + pJat8 (Registry)
*CW2553 + pJat8 (Registry)
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*DB3.1 (Invitrogen)
*DB3.1 (Invitrogen)
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**can propagate plasmids with ccdB toxin
 
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*DH5alpha (Invitrogen)
*DH5alpha (Invitrogen)
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**general purpose cloning
 
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**blue/white ok
 
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*KRX (Promega)
*KRX (Promega)
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**overexpresses LacI via the lacIq mutation on the F plasmid
 
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**can be used for autoinduction protocol (see Promega handbook)
 
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**blue/white ok
 
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*MC1061 (CGSC)
*MC1061 (CGSC)
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**no blue/white screening
 
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*MG1655 (CGSC)
*MG1655 (CGSC)
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**K-12 sequenced strain
 
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**no blue/white screening
 
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*MG1655-Z1 (Univ of Washington)
*MG1655-Z1 (Univ of Washington)
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**MG1655 with lacI- and tetR- overexpression cassettes in the genome, in the attB(lambda) locus
 
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*TOP10 (Invitrogen)
*TOP10 (Invitrogen)
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**general purpose cloning
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*W3110 (DSMZ)
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**blue/white ok
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*XL1-Blue (Stratagene)
*XL1-Blue (Stratagene)
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== Important glycerol stocks ==
 
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Current revision

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Antibiotics, inducers and substrates

  • Ampicillin - 100 mg/ml in deionized water (-20°C)
  • Chloramphenicol - 34 mg/ml in ethanol (-20°C)
  • Kanamycin - 50 mg/ml in deionized water (-20°C)
  • Gentamycin - 50 mg/ml in deionized water (-20°C)
  • G418 - 50 mg/ml in deionized water (+4°C)
  • HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
  • aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
  • IPTG Ready Made - 200 mM (-20°C)
  • X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

  • BW20767 (Registry)
  • BW23474 (CGSC)
  • BW25141 (CGSC)
  • BW25142 (CGSC)
  • CW2553 + pJat8 (Registry)
  • DB3.1 (Invitrogen)
  • DH5alpha (Invitrogen)
  • KRX (Promega)
  • MC1061 (CGSC)
  • MG1655 (CGSC)
  • MG1655-Z1 (Univ of Washington)
  • TOP10 (Invitrogen)
  • W3110 (DSMZ)
  • XL1-Blue (Stratagene)
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