Magni:Back Door

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Current revision (13:40, 28 March 2013) (view source)
(E. coli strains)
 
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*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
*Chloramphenicol - 34 mg/ml in ethanol (-20°C)
*Kanamycin - 50 mg/ml in deionized water (-20°C)
*Kanamycin - 50 mg/ml in deionized water (-20°C)
-
*HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C)
+
*Gentamycin - 50 mg/ml in deionized water (-20°C)
-
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol 70% or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
+
*G418 - 50 mg/ml in deionized water (+4°C)
 +
*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
 +
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
*IPTG Ready Made - 200 mM (-20°C)
*IPTG Ready Made - 200 mM (-20°C)
*X-Gal - 40 mg/ml in DMF (-20°C)
*X-Gal - 40 mg/ml in DMF (-20°C)
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*MG1655-Z1 (Univ of Washington)
*MG1655-Z1 (Univ of Washington)
*TOP10 (Invitrogen)
*TOP10 (Invitrogen)
 +
*W3110 (DSMZ)
*XL1-Blue (Stratagene)
*XL1-Blue (Stratagene)
-
 
-
== Important glycerol stocks ==
 
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Antibiotics, inducers and substrates

  • Ampicillin - 100 mg/ml in deionized water (-20°C)
  • Chloramphenicol - 34 mg/ml in ethanol (-20°C)
  • Kanamycin - 50 mg/ml in deionized water (-20°C)
  • Gentamycin - 50 mg/ml in deionized water (-20°C)
  • G418 - 50 mg/ml in deionized water (+4°C)
  • HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
  • aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
  • IPTG Ready Made - 200 mM (-20°C)
  • X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

  • BW20767 (Registry)
  • BW23474 (CGSC)
  • BW25141 (CGSC)
  • BW25142 (CGSC)
  • CW2553 + pJat8 (Registry)
  • DB3.1 (Invitrogen)
  • DH5alpha (Invitrogen)
  • KRX (Promega)
  • MC1061 (CGSC)
  • MG1655 (CGSC)
  • MG1655-Z1 (Univ of Washington)
  • TOP10 (Invitrogen)
  • W3110 (DSMZ)
  • XL1-Blue (Stratagene)
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