Magni:Back Door: Difference between revisions
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*Chloramphenicol - 34 mg/ml in ethanol (-20°C) | *Chloramphenicol - 34 mg/ml in ethanol (-20°C) | ||
*Kanamycin - 50 mg/ml in deionized water (-20°C) | *Kanamycin - 50 mg/ml in deionized water (-20°C) | ||
*HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C) | *Gentamycin - 50 mg/ml in deionized water (-20°C) | ||
*G418 - 50 mg/ml in deionized water (+4°C) | |||
*HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C) | |||
*aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE | *aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE | ||
*IPTG Ready Made - 200 mM (-20°C) | *IPTG Ready Made - 200 mM (-20°C) | ||
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*MG1655-Z1 (Univ of Washington) | *MG1655-Z1 (Univ of Washington) | ||
*TOP10 (Invitrogen) | *TOP10 (Invitrogen) | ||
*W3110 (DSMZ) | |||
*XL1-Blue (Stratagene) | *XL1-Blue (Stratagene) | ||
</div> | </div> | ||
Revision as of 10:40, 28 March 2013
Antibiotics, inducers and substrates
- Ampicillin - 100 mg/ml in deionized water (-20°C)
- Chloramphenicol - 34 mg/ml in ethanol (-20°C)
- Kanamycin - 50 mg/ml in deionized water (-20°C)
- Gentamycin - 50 mg/ml in deionized water (-20°C)
- G418 - 50 mg/ml in deionized water (+4°C)
- HSL (N-3-oxo-C6-L-homoserine lactone) - 2 mM in water (-20°C)
- aTc (Anhydrotetracycline) - 2 mg/ml in ethanol or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
- IPTG Ready Made - 200 mM (-20°C)
- X-Gal - 40 mg/ml in DMF (-20°C)
E. coli strains
- BW20767 (Registry)
- BW23474 (CGSC)
- BW25141 (CGSC)
- BW25142 (CGSC)
- CW2553 + pJat8 (Registry)
- DB3.1 (Invitrogen)
- DH5alpha (Invitrogen)
- KRX (Promega)
- MC1061 (CGSC)
- MG1655 (CGSC)
- MG1655-Z1 (Univ of Washington)
- TOP10 (Invitrogen)
- W3110 (DSMZ)
- XL1-Blue (Stratagene)
