Magni:Data

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|[[Image:constRPUlow.png|thumb|left|Steady-state characterization in microplate reader for 8 promoters via the BBa_I13507 (RFP34) reporter system in pSB4C5 in the KRX (Promega) strain, M9 suppl medium (Pasotti et al., PLoS ONE, 2012)]]
|[[Image:constRPUlow.png|thumb|left|Steady-state characterization in microplate reader for 8 promoters via the BBa_I13507 (RFP34) reporter system in pSB4C5 in the KRX (Promega) strain, M9 suppl medium (Pasotti et al., PLoS ONE, 2012)]]
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|[[Image:chemicalinducersHL.png|thumb|left|Degradation rate of 3 chemical inducers commonly used for recombinant gene expression in different conditions. The k values (1/h) are the result of a first-order exponential fitting of data from a 32-h experiment. 95% confidence intervals are in brackets. Cultured broth conditions are with the MG1655-Z1 strain, inoculated from a single colony at t=0. (Politi et al.)]]
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|[[Image:chemicalinducersHL.png|thumb|left|Degradation rate of ATc, HSL and IPTG in different conditions. k (1/h) is the result of a monoexponential fitting of data from a 32-h experiment. 95% confidence intervals are in brackets. Cultured broth conditions are with the MG1655-Z1 strain, inoculated from a single colony at t=0. IPTG is apparently degraded in cultured broths, but it disappears for the uptake by LacY, not for a decay process. (Politi et al.)]]
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Revision as of 05:21, 8 December 2013

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Insert datasheets or representative experiments in this page.


Datasheet produced for BBa_K112808 (Pasotti et al., J Biol Eng, 2011)
Datasheet produced for BBa_K112808 (Pasotti et al., J Biol Eng, 2011)
Image:F2620absrel.png
Steady-state characterization in microplate reader of BBa_F2620 in absolute and relative units in the MG1655 strain, M9 suppl medium (Zucca et al., BMC Bioinformatics, 2012)
Image:ConstRPUplos.png
Steady-state characterization in microplate reader for 5 promoters via 3 different reporter systems: BBa_E0240 (GFP32), BBa_I13507 (RFP34) and BBa_K516032 (RFP32), all in pSB4C5 in the TOP10 (Invitrogen) strain. Upper panel: M9 suppl medium, lower panel: LB medium. PLlacO1 is induced with 1mM IPTG (Pasotti et al., PLoS ONE, 2012)
Image:ConstRPUlow.png
Steady-state characterization in microplate reader for 8 promoters via the BBa_I13507 (RFP34) reporter system in pSB4C5 in the KRX (Promega) strain, M9 suppl medium (Pasotti et al., PLoS ONE, 2012)
Image:ChemicalinducersHL.png
Degradation rate of ATc, HSL and IPTG in different conditions. k (1/h) is the result of a monoexponential fitting of data from a 32-h experiment. 95% confidence intervals are in brackets. Cultured broth conditions are with the MG1655-Z1 strain, inoculated from a single colony at t=0. IPTG is apparently degraded in cultured broths, but it disappears for the uptake by LacY, not for a decay process. (Politi et al.)
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