Magni:Protocols

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(Plasmid construction)
(Plasmid construction)
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= Plasmid construction =
= Plasmid construction =
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*[[Magni:Protocols/Competentcells|Competent cells]]
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]]
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]]
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=== 1 ===
+
*[[Magni:Protocols/Transformation|Transformation]]
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=== 2 ===
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*[[Magni:Protocols/Miniprep|Miniprep]]
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*[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]]
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*[[Magni:Protocols/Electrophoresis|Electrophoresis]]
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*[[Magni:Protocols/gelextraction|Gel extraction]]
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*[[Magni:Protocols/purificationkit|DNA purification (with columns)]]
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*[[Magni:Protocols/sodiumacetate|DNA purification via ethanol precipitation with sodium acetate]]
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*[[Magni:Protocols/Ligation|Ligation]]
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*[[Magni:Protocols/PCR|PCR]]
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*[[Magni:Protocols/phosphatase|DNA dephosphorylation with Antarctic Phosphatase]]
= Characterization =
= Characterization =

Revision as of 10:22, 30 July 2012

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Contents

Plasmid construction

Characterization

1

2

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