Magni:Protocols: Difference between revisions
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*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]] | *[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]] | ||
*[[Magni:Protocols/Transformation|Transformation]] | *[[Magni:Protocols/Transformation|Transformation]] | ||
*[[Magni:Protocols/Inocula|Plasmid propagation ''in vivo'']] | |||
*[[Magni:Protocols/Miniprep|Miniprep]] | *[[Magni:Protocols/Miniprep|Miniprep]] | ||
*[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]] | *[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]] | ||
Revision as of 07:24, 30 July 2012
Plasmid construction
- Competent cells
- Plasmid resuspension from iGEM kit plates
- Transformation
- Plasmid propagation in vivo
- Miniprep
- BioBrick digestion with restriction enzymes
- Electrophoresis
- Gel extraction
- DNA purification (with columns)
- DNA purification via ethanol precipitation with sodium acetate
- Ligation
- PCR
- DNA dephosphorylation with Antarctic Phosphatase
