Magni:Protocols

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(Plasmid construction)
Current revision (02:49, 15 July 2013) (view source)
(Characterization)
 
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= Growth media =
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*[[Magni:Protocols/LB|LB]]
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*[[Magni:Protocols/M9|M9]]
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*[[Magni:Protocols/ph|pH adjustment for LB or M9]]
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*[[Magni:Protocols/buffering|Buffered media]]
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*[[Magni:Protocols/staining|Blue-white screening on plates]]
= Plasmid construction =
= Plasmid construction =
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*[[Magni:Protocols/Competentcells|Competent cells]]
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]]
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]]
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=== 1 ===
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*[[Magni:Protocols/Transformation|Transformation]]
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=== 2 ===
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*[[Magni:Protocols/Inocula|Plasmid propagation]]
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*[[Magni:Protocols/Miniprep|Miniprep]]
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*[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]]
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*[[Magni:Protocols/Electrophoresis|Electrophoresis]]
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*[[Magni:Protocols/gelextraction|Gel extraction]]
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*[[Magni:Protocols/purificationkit|DNA purification (with columns)]]
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*[[Magni:Protocols/sodiumacetate|DNA purification via ethanol precipitation with sodium acetate]]
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*[[Magni:Protocols/Ligation|Ligation]]
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*[[Magni:Protocols/PCR|PCR]]
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*[[Magni:Protocols/phosphatase|DNA dephosphorylation with Antarctic Phosphatase]]
= Characterization =
= Characterization =
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=== 1 ===
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*[[Magni:Protocols/tecan|Assays with Tecan Infinite F200 microplate reader]]
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=== 2 ===
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*[[Magni:Protocols/fermentation|Fermentation]]
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*[[Magni:Protocols/chemostat|Chemostat]]
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*[[Magni:Protocols/flowcy|Preparation of samples for flow cytometry]]
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*[[Magni:Protocols/hplc|Preparation of samples for HPLC]]
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Current revision

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Growth media

Plasmid construction

Characterization

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