Magni:Protocols: Difference between revisions
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= Plasmid construction = | = Plasmid construction = | ||
*[[Magni:Protocols/Competentcells|Competent cells]] | |||
*[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]] | *[[Magni:Protocols/Resuspension|Plasmid resuspension from iGEM kit plates]] | ||
*[[Magni:Protocols/Transformation|Transformation]] | |||
*[[Magni:Protocols/Miniprep|Miniprep]] | |||
*[[Magni:Protocols/Digestion|BioBrick digestion with restriction enzymes]] | |||
*[[Magni:Protocols/Electrophoresis|Electrophoresis]] | |||
*[[Magni:Protocols/gelextraction|Gel extraction]] | |||
*[[Magni:Protocols/purificationkit|DNA purification (with columns)]] | |||
*[[Magni:Protocols/sodiumacetate|DNA purification via ethanol precipitation with sodium acetate]] | |||
*[[Magni:Protocols/Ligation|Ligation]] | |||
*[[Magni:Protocols/PCR|PCR]] | |||
*[[Magni:Protocols/phosphatase|DNA dephosphorylation with Antarctic Phosphatase]] | |||
= Characterization = | = Characterization = | ||
Revision as of 07:22, 30 July 2012
Plasmid construction
- Competent cells
- Plasmid resuspension from iGEM kit plates
- Transformation
- Miniprep
- BioBrick digestion with restriction enzymes
- Electrophoresis
- Gel extraction
- DNA purification (with columns)
- DNA purification via ethanol precipitation with sodium acetate
- Ligation
- PCR
- DNA dephosphorylation with Antarctic Phosphatase
