Magni:Protocols: Difference between revisions
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= Characterization = | = Characterization = | ||
*[[Magni:Protocols/tecan|Assays with Tecan Infinite F200 microplate reader]] | *[[Magni:Protocols/tecan|Assays with Tecan Infinite F200 microplate reader]] | ||
*[[Magni:Protocols/fermentation|Fermentation]] | |||
*[[Magni:Protocols/flowcy|Preparation of samples for flow cytometry]] | |||
</div> | </div> | ||
Revision as of 10:45, 28 March 2013
Growth media
Plasmid construction
- Competent cells
- Plasmid resuspension from iGEM kit plates
- Transformation
- Plasmid propagation
- Miniprep
- BioBrick digestion with restriction enzymes
- Electrophoresis
- Gel extraction
- DNA purification (with columns)
- DNA purification via ethanol precipitation with sodium acetate
- Ligation
- PCR
- DNA dephosphorylation with Antarctic Phosphatase
