Revision as of 12:14, 30 July 2012

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LB medium (liquid)

For every 1 L of medium, add to a pyrex bottle or flask:
- 10 g NaCl
- 10 g bacto-tryptone
- 5 g yeast extract
Add 1 L of deionized water (blue tap of the Millipore machine).
Autoclave (if using a bottle, do not close the cap completely).
Let it cool to about 40degC and, when required, add antibiotic near the flame.
Store in the fridge and always check for contaminations before using.

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-= LB medium =
+= LB medium (liquid) =
-*ff
+*For every 1 L of medium, add to a pyrex bottle or flask:
-*dd
+**10 g NaCl
+**10 g bacto-tryptone
+**5 g yeast extract
+*Add 1 L of deionized water (blue tap of the Millipore machine).
+*Autoclave (if using a bottle, do not close the cap completely).
+*Let it cool to about 40degC and, when required, add antibiotic near the flame.
+*Store in the fridge and ''always'' check for contaminations before using.
 </div>