Magni:Protocols/Miniprep: Difference between revisions
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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Miniprep = *Follow the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the exception that elut...) |
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* | *Use the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the following exceptions: | ||
** saturated bacterial culture can be centrifuged at 4000 rpm, 4degC, 10 min; | |||
** elution step can be done with 30 ul of Milli-Q water (from the filter of the Millipore machine). | |||
Latest revision as of 13:51, 9 September 2012
Miniprep
- Use the NucleoSpin Plasmid kit (Macherey-Nagel) instructions, with the following exceptions:
- saturated bacterial culture can be centrifuged at 4000 rpm, 4degC, 10 min;
- elution step can be done with 30 ul of Milli-Q water (from the filter of the Millipore machine).
