Magni:Protocols/PCR

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Lorenzo Pasotti (Talk | contribs)
(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = PCR = *Follow the NEB Phusion Hot Start Flex DNA Polymerase instructions. DNA can be from a miniprep of f...)
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PCR

  • Follow the NEB Phusion Hot Start Flex DNA Polymerase instructions. DNA can be from a miniprep of from a colony. If assaying a colony, one can: i) pick a colony, resuspend it into 20 ul of water/LB and pipette 1 ul into the PCR mix (recommended) or ii) simply "infect" the PCR mix with the picked colony by pipetting up and down.
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