From OpenWetWare

< Magni:Protocols
Revision as of 13:51, 22 September 2012 by Lorenzo Pasotti (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


  • Follow the NEB Phusion Hot Start Flex DNA Polymerase instructions. DNA can be from a miniprep of from a colony. If assaying a colony, one can: i) pick a colony, resuspend it into 20 ul of water/LB and pipette 1 ul into the PCR mix (recommended) or ii) simply "infect" the PCR mix with the picked colony by pipetting up and down.
Personal tools