Magni:Protocols/gelextraction

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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Gel extraction = *Set the transilluminator to 70% of power, prepare a becker with deionized water, a clea...)
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*Cut the band of interest and put it into an empty labelled 1.5-ml tube.
*Cut the band of interest and put it into an empty labelled 1.5-ml tube.
*Follow the NucleoSpin Extract II kit (Macherey-Nagel) with the exception that elution can be performed with 30 ul of Milli-Q water.
*Follow the NucleoSpin Extract II kit (Macherey-Nagel) with the exception that elution can be performed with 30 ul of Milli-Q water.
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SAFETY NOTES (the same as electrophoresis):
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* ethidium bromide is toxic and mutagenic and is a suspected carcinogen. Be careful when handling ethidium bromide solutions and gel (always with gloves).
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* ultraviolet radiation is harmful to the skin and eyes. Wear gloves and a face shield when using an exposed ultraviolet source. Always use the transilluminator protection. SKIN MUST NOT BE EXPOSED TO UV!!!

Revision as of 14:35, 22 September 2012

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Gel extraction

  • Set the transilluminator to 70% of power, prepare a becker with deionized water, a clean scalpel and the suitable protections for UV.
  • Cut the band of interest and put it into an empty labelled 1.5-ml tube.
  • Follow the NucleoSpin Extract II kit (Macherey-Nagel) with the exception that elution can be performed with 30 ul of Milli-Q water.

SAFETY NOTES (the same as electrophoresis):

  • ethidium bromide is toxic and mutagenic and is a suspected carcinogen. Be careful when handling ethidium bromide solutions and gel (always with gloves).
  • ultraviolet radiation is harmful to the skin and eyes. Wear gloves and a face shield when using an exposed ultraviolet source. Always use the transilluminator protection. SKIN MUST NOT BE EXPOSED TO UV!!!
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