Magni:Protocols/gelextraction

From OpenWetWare

< Magni:Protocols(Difference between revisions)
Jump to: navigation, search
Current revision (14:36, 22 September 2012) (view source)
 
Line 8: Line 8:
SAFETY NOTES (the same as electrophoresis):
SAFETY NOTES (the same as electrophoresis):
-
* ethidium bromide is toxic and mutagenic and is a suspected carcinogen. Be careful when handling ethidium bromide solutions and gel (always with gloves).
+
* ethidium bromide is toxic and mutagenic and is a suspected carcinogen. Be careful when handling ethidium bromide solutions and gel pieces (always with gloves).
-
* ultraviolet radiation is harmful to the skin and eyes. Wear gloves and a face shield when using an exposed ultraviolet source. Always use the transilluminator protection. SKIN MUST NOT BE EXPOSED TO UV!!!
+
* ultraviolet radiation is harmful to the skin and eyes. Wear gloves and a face shield when using an exposed ultraviolet source. Always use the transilluminator protection. Skin must not be exposed, use lab coat and gloves during gel cut.

Current revision

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


Gel extraction

  • Set the transilluminator to 70% of power, prepare a becker with deionized water, a clean scalpel and the suitable protections for UV.
  • Cut the band of interest and put it into an empty labelled 1.5-ml tube.
  • Follow the NucleoSpin Extract II kit (Macherey-Nagel) with the exception that elution can be performed with 30 ul of Milli-Q water.

SAFETY NOTES (the same as electrophoresis):

  • ethidium bromide is toxic and mutagenic and is a suspected carcinogen. Be careful when handling ethidium bromide solutions and gel pieces (always with gloves).
  • ultraviolet radiation is harmful to the skin and eyes. Wear gloves and a face shield when using an exposed ultraviolet source. Always use the transilluminator protection. Skin must not be exposed, use lab coat and gloves during gel cut.
Personal tools