Magni:Protocols/hplc: Difference between revisions
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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Preparation of samples for HPLC = *Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20...) |
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*Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C. | *Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C. | ||
*The storage of two samples is recommended for backup reasons. | *The storage of two samples is recommended for backup reasons. | ||
*To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler. | *To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler. | ||
*At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample in an "insert" inside a vial. | |||
</div> | </div> | ||
Latest revision as of 07:15, 5 May 2013
Preparation of samples for HPLC
- Filter-sterilize (0.2 um) samples and store them in 2-ml tubes at -20°C.
- The storage of two samples is recommended for backup reasons.
- To start analysis, transfer the content of a 2-ml tube into a vial, seal the cap and put the vial in the auto-sampler.
- At least 800 ul are required for each HPLC analysis. If this volume is not available, store at least 150 ul and analyze sample in an "insert" inside a vial.
