Magni:Protocols/ph

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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = pH adjustment for LB medium = *Before autoclaving the LB medium: ** dip pH-meter probe in the medium to m...)
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Revision as of 14:08, 28 March 2013

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pH adjustment for LB medium

  • Before autoclaving the LB medium:
    • dip pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
    • ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
    • wash the pH probe with deionized water and store it in KCl 3M.
  • Autoclave the medium
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