Magni:Protocols/ph

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = pH adjustment for LB medium = *Before autoclaving the LB medium: ** dip pH-meter probe in the medium to m...)
Line 4: Line 4:
= pH adjustment for LB medium =
= pH adjustment for LB medium =
*Before autoclaving the LB medium:
*Before autoclaving the LB medium:
-
** dip pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
+
** dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
** wash the pH probe with deionized water and store it in KCl 3M.
** wash the pH probe with deionized water and store it in KCl 3M.
* Autoclave the medium
* Autoclave the medium
 +
 +
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
 +
= pH adjustment for M9 medium =
 +
*According to the M9 medium preparation protocol, mix:
 +
**water,
 +
**glycerol and
 +
**M9 salts.
 +
**Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
 +
** ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
 +
** wash the pH probe with deionized water and store it in KCl 3M.
 +
* Autoclave the mix in a bottle.
 +
* Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4).
</div>
</div>

Revision as of 11:43, 18 April 2013

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


pH adjustment for LB medium

  • Before autoclaving the LB medium:
    • dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
    • ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
    • wash the pH probe with deionized water and store it in KCl 3M.
  • Autoclave the medium

pH adjustment for M9 medium

  • According to the M9 medium preparation protocol, mix:
    • water,
    • glycerol and
    • M9 salts.
    • Dip the pH-meter probe in the medium to monitor pH (wash probe with deionized water and calibrate pH-meter with 2 points when required);
    • ajust pH with HCl, NaOH (or KOH). While handling strong acid/base, always wear suitable protections!
    • wash the pH probe with deionized water and store it in KCl 3M.
  • Autoclave the mix in a bottle.
  • Equilibrate at room temperature and add the remaining ingredients (casamino acids, thiamine, CaCl2, MgSO4).
Personal tools