Magni:Protocols/sodiumacetate

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = DNA purification (sodium acetate) = *)
Line 3: Line 3:
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
<div style="padding: 10px; width: 720px; border: 5px solid #000000;">
= DNA purification (sodium acetate) =
= DNA purification (sodium acetate) =
-
*
+
*Add 1/10 of the original volume of sodium acetate 3 M
 +
*Add 2.5-fold of the original volume of absolute ethanol
 +
*Freeze at -80degC for 30 min or at -20degC overnight
 +
*Centrifuge at 13000 rpm, 4degC for 20 min
 +
*Decant supernatant
 +
*Add 250 µl of 70% ethanol
 +
*Centrifuge at 13000 rpm, 4degC for 20 min
 +
*Remove all supernatant with a pipette
 +
*Air dry pellet until ethanol is totally removed
 +
*Elute with 5-10 ul Milli-Q water

Revision as of 13:32, 22 September 2012

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       


DNA purification (sodium acetate)

  • Add 1/10 of the original volume of sodium acetate 3 M
  • Add 2.5-fold of the original volume of absolute ethanol
  • Freeze at -80degC for 30 min or at -20degC overnight
  • Centrifuge at 13000 rpm, 4degC for 20 min
  • Decant supernatant
  • Add 250 µl of 70% ethanol
  • Centrifuge at 13000 rpm, 4degC for 20 min
  • Remove all supernatant with a pipette
  • Air dry pellet until ethanol is totally removed
  • Elute with 5-10 ul Milli-Q water
Personal tools