Maheshri:Duties

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Long-term Duties

EHS Rep

Currently: Shawn Finney-Manchester

Computer Guru

Currently: C.J. Zopf

Web/Wiki Master

Currently: T.L. To

Maintenance

Currently: Tek Hyung Lee

Inventory

Currently: Katie Quinn

Social Chair

Currently: Bradley Niesner

Rotating Duties (2-month rotation)

Ordering/Autoclave Biological Waste/Autoclave Validation

Headline text

• Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board.
• When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least).
• Once a week, the following stocks need to be checked and any low or missing items should be ordered.

Item                                        Location
Pipettor tips (10uL, 200uL, 1000uL)         Glass cabinet near window
Filtered Pipettor tips (200uL, 1000uL)      Above Glass cabinet near window
1.5mL Microcentrifuge tubes                 Glass cabinet near window
Blue cap Falcon tubes (15mL, 50mL)          Glass cabinet near window
Test tubes (13x100mm, 16x150mm)             Glass cabinet near window
PCR strip tubes                             Glass cabinet near window
Syringe filters (0.2um, 0.45um)             Glass cabinet near window
Plastic syringes (10-50mL)                  Drawers under media station
Nalgene vacuum filters and bottles          Above glass cabinet near window
Gloves                                      Glass cabinet under liquid media
Seriological Pipettes (5mL, 10mL, 25mL)     Cabinet under balances
Petri Dishes                                Cabinet under Bradley’s bench
Phenol-Chloroform-Isoamyl Alcohol           Small 4ºC refrigerator
200 Proof Ethanol                           Solvent cabinet under right fume hood
Razors                                      Above gel viewer

• Common enzymes should also be checked once a week. The enzyme boxes in the -20 are labeled with all comon enzymes on the lid. If we are out of an enzyme, its well will be empty. Any duplicate unopened enzymes should remain in their packages and be placed next to the enzyme boxes.

Liquid media

Common media and solutions to make and autoclave:

• YePD
• 2xYPAD
• TB
• SD min
• 50% glucose solution
• ddH2O

Plates

Common media plates to make:

• TB-Amp
• YePD plates

Autoclaving small items

Autoclave and stock shelves:

• microcentrifuge tubes
• toothpicks
• applicators
• 96-well deep well plates

• pipette tips (10μL, 200μL, 1000μL) <== separate duty

Dishwashing

Either by hand or using dishwasher in the autoclave room, wash the common dishes:

• Media/solution bottles and caps
• Erlenmeyer flasks
• Test tube caps

Autoclaving culture tubes and flasks

Autoclave to restock sterile:

• Test tubes with clean caps (13x100mm blue cap, 16x150mm green cap)
• 250mL Erlenmeyer flasks with aluminum foil caps

Autoclaving biological waste

http://openwetware.org/wiki/Maheshri:SOPs#Waste_Management_Operating_Procedures