Maheshri:Duties: Difference between revisions
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*Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board. | *Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board. | ||
*When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least). | *When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least). | ||
*Once a week, the following stocks need to be checked and any low or missing items should be ordered. A list of commonly ordered items and their VWR catalog numbers is available [ | *Once a week, the following stocks need to be checked and any low or missing items should be ordered. A list of commonly ordered items and their VWR catalog numbers is available [http://openwetware.org/wiki/Maheshri:Locations here] | ||
'''Item''' '''Location''' | '''Item''' '''Location''' |
Revision as of 11:35, 14 June 2010
Long-term Duties
EHS Rep
Currently: Shawn Finney-Manchester
Computer Guru
Currently: C.J. Zopf
Web/Wiki Master
Currently: T.L. To
Maintenance
Currently: Tek Hyung Lee
Inventory
Currently: Katie Quinn
Social Chair
Currently: Bradley Niesner
Rotating Duties (2-month rotation)
Ordering/Autoclave Biological Waste/Autoclave Validation
Ordering
- Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board.
- When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least).
- Once a week, the following stocks need to be checked and any low or missing items should be ordered. A list of commonly ordered items and their VWR catalog numbers is available here
Item Location Pipettor tips (10uL, 200uL, 1000uL) Glass cabinet near window Filtered Pipettor tips (200uL, 1000uL) Above Glass cabinet near window 1.5mL Microcentrifuge tubes Glass cabinet near window Blue cap Falcon tubes (15mL, 50mL) Glass cabinet near window Test tubes (13x100mm, 16x150mm) Glass cabinet near window PCR strip tubes Glass cabinet near window Syringe filters (0.2um, 0.45um) Glass cabinet near window Plastic syringes (10-50mL) Drawers under media station Nalgene vacuum filters and bottles Above glass cabinet near window Gloves Glass cabinet under liquid media Seriological Pipettes (5mL, 10mL, 25mL) Cabinet under balances Petri Dishes Cabinet under Bradley’s bench Phenol-Chloroform-Isoamyl Alcohol Small 4ºC refrigerator 200 Proof Ethanol Solvent cabinet under right fume hood Razors Above gel viewer
- Common enzymes should also be checked once a week. The enzyme boxes in the -20 are labeled with all comon enzymes on the lid. If we are out of an enzyme, its well will be empty. Any duplicate unopened enzymes should remain in their packages and be placed next to the enzyme boxes.
Liquid media
Common media and solutions to make and autoclave:
- YePD
- 2xYPAD
- TB
- SD min
- 50% glucose solution
- ddH2O
Plates
Common media plates to make:
- TB-Amp
- YePD plates
Autoclaving small items
Autoclave and stock shelves:
- microcentrifuge tubes
- toothpicks
- applicators
- 96-well deep well plates
- pipette tips (10μL, 200μL, 1000μL) <== separate duty
Dishwashing
Either by hand or using dishwasher in the autoclave room, wash the common dishes:
- Media/solution bottles and caps
- Erlenmeyer flasks
- Test tube caps
Autoclaving culture tubes and flasks
Autoclave to restock sterile:
- Test tubes with clean caps (13x100mm blue cap, 16x150mm green cap)
- 250mL Erlenmeyer flasks with aluminum foil caps
Autoclaving biological waste
http://openwetware.org/wiki/Maheshri:SOPs#Waste_Management_Operating_Procedures