Maheshri:Duties

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Long-term Duties

EHS Rep

Currently: Katie Quinn

Computer Guru

Currently: Nick Wren

Web/Wiki Master

Currently: Tek Hyung Lee

Maintenance

Currently: Tek Hyung Lee

Inventory & Microscope

Currently: C.J. Zopf

Social Chair

Currently: Bradley Niesner

Rotating Duties (2-month rotation)

Ordering/Autoclave Biological Waste

Ordering

• Every day, items that are written on the white board in the office should be ordered and a check placed next to the item. When the item arrives, erase the item from the board.
• When items arrive, stock the shelves and bring the packing slip to the Administrative Services Office in 8-328 (weekly at least).
• Once a week, the following stocks need to be checked and any low or missing items should be ordered. A printable list of the commonly ordered items below and their VWR catalog numbers is available here.
• For urgent supplies, any catalog number followed by "SR" can be purchased in the stockroom in 56-070 (though the catalog numbers often represent bulk packages, the stockroom usually has smaller quantities or different brands).

Lab Supplies

Name	Location	VWR Catalog # (SR)	Reorder?
Filter pipettor tips (1mL)	Above cupboard	10011-350 (SR)
Filter pipettor tips (200μL)	Above cupboard	10011-356 (SR)
MF75 Vacuum Filter Units (500mL)	Above cupboard	28199-177
MF75 Vacuum Filter Units (250mL)	Above cupboard	28199-123
Microtube (1.5mL)	Cupboard	10011-700 (SR)
Pipettor tips (1mL)	Cupboard	89100-006 (SR)
Pipettor tips (300μL)	Cupboard	89100-002 (SR)
Pipettor tips (10μL)	Cupboard	89100-000 (SR)
Test tube (13x100mm)	Cupboard	47729-572
Test tube (16x125mm)	Cupboard	47729-578
Falcon tube (50mL)	Cupboard	21008-951 (SR)
Falcon tube (15mL)	Cupboard	21008-918 (SR)
Syringe filter (0.45μm)	Cupboard	28145-481 (SR)
PCR strip tubes	Cupboard	12000-206
Reservoir for multi-channel pipettor	Cupboard	89094-676
Cryogenic vials	Cupboard	66021-974 (SR)
Gloves (Large blue)	Cupboard	89094-676 (SR)
Gloves (Large black)	Cupboard	94001-370 (SR)
Gloves (Medium black)	Cupboard	94001-368 (SR)
Gloves (Small black)	Cupboard	94001-366 (SR)
Freezer storage boxes	Side shelf	82007-162 (SR)
Plastic syringes (10mL)	Side shelf	BD309604
Plastic syringes (30mL)	Side shelf	BD309650
Serological pipet (25mL)	Media station cabinet	53300-567
Serological pipet (10mL)	Media station cabinet	357551
Serological pipet (5mL)	Media station cabinet	53300-421
Petri dishes (100x15mm)	Left window bench cabinet	25384-094 (SR)
200 Proof EtOH (Solvent Room E25-169 10:30PM~12:00PM)	Under fume hood 2	EM-EX0276-3S (SolventR)
Phenol-Chloroform-Isoamyl Alcohol	Small 4ºC refrigerator	PI17908 (SR)
Aluminum foil	Shelf next to the whiteboard	89079-070 (SR)
Plastic wrap	Shelf next to the whiteboard	89136-664 (SR)
Razors	Shelf next to the whiteboard	55411-050 (SR)
Common enzymes (HF preferred)	-20C freezer

• Common enzymes should also be checked once a week, and most can be purchased through the stockroom. The enzyme boxes in the -20 are labeled with all common enzymes on the lid. If we are out of an enzyme, its well will be empty. Any duplicate unopened enzymes should remain in their packages and be placed next to the enzyme boxes. Order the HF (high fidelity) version of the enzyme if it exists to facilitate double digests.

Autoclave Biological Waste

The non-sharp biological waste bags need to be removed from their bins and autoclaved before they can be thrown in the regular trash. See the protocol here

Liquid media

Common media and solutions to make and autoclave:

• YePD
• 2xYPAD
• TB
• SD min
• 50% glucose solution
• ddH2O

Plates

Common media plates to make:

• TB-Amp
• YePD plates

Autoclaving tips

Autoclave and stock shelves

• pipette tips (10μL, 200μL, 1000μL)

Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry

Dishwashing

Either by hand or using dishwasher in the autoclave room, wash the common dishes:

• Media/solution bottles and caps
• Erlenmeyer flasks
• Test tube caps

Autoclaving culture tubes and flasks

Autoclave to restock sterile:

• Test tubes with clean caps (13x100mm blue cap, 16x150mm green cap)
• 250mL Erlenmeyer flasks with aluminum foil caps

Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry

Autoclaving small items

Autoclave and stock shelves:

• microcentrifuge tubes
• toothpicks
• applicators
• 96-well deep well plates

Autoclaving can be done on dry cycle: 20 min sterilize/20 min dry