Maheshri:Internal: Difference between revisions
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*[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | *[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | ||
*[[Maheshri:Competent| Making Chemically Competent Bacteria]] | *[[Maheshri:Competent| Making Chemically Competent Bacteria]] | ||
*[[Maheshri:CompetentE| Making Electro-competent Bacteria]] | |||
*[[Maheshri:TransformationB| Bacterial Transformation]] | *[[Maheshri:TransformationB| Bacterial Transformation]] | ||
*[[Maheshri:TransformationY| Yeast Transformation]] | *[[Maheshri:TransformationY| Yeast Transformation]] | ||
*[[Maheshri:Miniprep| Bacterial Plasmid Miniprep]] | *[[Maheshri:Miniprep| Bacterial Plasmid Miniprep]] | ||
*[[Maheshri:TransformationYE| Yeast Transformation by Electroporation]] | |||
*[[Maheshri:GenomicPrep| Yeast Genomic Prep]] | *[[Maheshri:GenomicPrep| Yeast Genomic Prep]] | ||
*[[Maheshri:MiniprepY| Yeast Plasmid Prep]] | *[[Maheshri:MiniprepY| Yeast Plasmid Prep]] | ||
Revision as of 14:47, 5 September 2009
General Information
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Common Area Etiquette
- Locations of Common Items and Reagents
- Restriction Enzymes
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- Freezing Cells for Long-term Storage
- PCR
- Kinase and Anneal Oligos
- Restriction Digestion
- Agarose Gel Electrophoresis
- DNA Ligation (OpenWetWare)
- Making Chemically Competent Bacteria
- Making Electro-competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Transformation by Electroporation
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
