Maheshri:Internal: Difference between revisions
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*[[Maheshri:Duties | Lab Duties]] | *[[Maheshri:Duties | Lab Duties]] | ||
*[[Maheshri:SOPs | Standard Operating Procedures for the Equipment]] | *[[Maheshri:SOPs | Standard Operating Procedures for the Equipment]] | ||
*[[Maheshri:SOPs-prot | Standard Operating Procedures for the Protocol]] | |||
*[[Maheshri:CommonArea | Common Area Etiquette]] | *[[Maheshri:CommonArea | Common Area Etiquette]] | ||
*[[Maheshri:Locations | Locations of Common Items and Reagents]] | *[[Maheshri:Locations | Locations of Common Items and Reagents]] | ||
*[[Maheshri:Enzymes| | *[[Maheshri:Enzymes| Enzymes]] | ||
*[[Maheshri:Abbreviations| Abbreviations]] | |||
*[[Maheshri:Equipment| Equipment]] | |||
*[[Maheshri:Computing| Computing]] | *[[Maheshri:Computing| Computing]] | ||
| Line 16: | Line 19: | ||
*[[Maheshri:Culturing| Culturing Yeast]] | *[[Maheshri:Culturing| Culturing Yeast]] | ||
*[[Maheshri:Mating| Non-selective Mating of Yeast]] | *[[Maheshri:Mating| Non-selective Mating of Yeast]] | ||
*[[Maheshri: | *[[Maheshri:Liquid Sporulation| Sporulation in Liquid Media]] | ||
*[[Maheshri:PCR| PCR]] | *[[Maheshri:PCR| PCR]] | ||
*[[Maheshri:Kinase| Kinase and Anneal Oligos]] | *[[Maheshri:Kinase| Kinase and Anneal Oligos]] | ||
*[[Maheshri:Digestion| Restriction Digestion]] | *[[Maheshri:Digestion| Restriction Digestion]] | ||
*[ | *[[Maheshri:Gel| Agarose gel electrophoresis ]] | ||
*[[Maheshri:GelEx| Gel Extraction]] | |||
*[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | *[http://openwetware.org/wiki/DNA_Ligation DNA Ligation (OpenWetWare)] | ||
*[[Maheshri:Competent| Making Chemically Competent Bacteria]] | *[[Maheshri:Competent| Making Chemically Competent Bacteria]] | ||
| Line 38: | Line 42: | ||
*[[Maheshri:RTPCR| RT-PCR]] | *[[Maheshri:RTPCR| RT-PCR]] | ||
*[[Maheshri:FluorescenceM | Fluorescence Microscopy]] | *[[Maheshri:FluorescenceM | Fluorescence Microscopy]] | ||
*[[Maheshri:3C | Cromatin Conformation Capture (3C)]] | |||
*[[Maheshri:Microfluidics | Microfluidic Experiments]] | |||
=Software= | =Software= | ||
*[http://ist.mit.edu/services/software/matlab MATLAB] | |||
*VectorNTI; speak to the Computer Guru about obtaining a copy. [http://www.invitrogen.com/site/us/en/home/LINNEA-Online-Guides/LINNEA-Communities/Vector-NTI-Community/vector-nti-software/VNTI-Tutorials.html VectorNTI tutorials & reference guide] | |||
*[[Maheshri:Software | Cell tracking and analysis]] for time-lapse fluorescent microscopy | |||
=Databases= | |||
*[[Maheshri:Chemical Inventory | Chemical Inventory]] | |||
=Useful Links= | =Useful Links= | ||
*[http://www.yeastgenome.org/ Saccharomyces Genome Database (SGD)] | |||
*[http://www.ncbi.nlm.nih.gov/ NCBI Homepage] | |||
*[http://www.kazusa.or.jp/codon/ Codon Usage] | |||
*[http://jaligner.sourceforge.net/ Biological Sequence Alignment] | |||
*[http://mrw.interscience.wiley.com/emrw/9780471142720/home/ Current Protocols in Molecular Biology] | *[http://mrw.interscience.wiley.com/emrw/9780471142720/home/ Current Protocols in Molecular Biology] | ||
*[http://dbb.urmc.rochester.edu/labs/sherman_f/StartedYeast.html Getting Started with Yeast] | *[http://dbb.urmc.rochester.edu/labs/sherman_f/StartedYeast.html Getting Started with Yeast] | ||
*[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp NEB Double Digest Finder] | *[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp NEB Double Digest Finder] | ||
*[http://www.microscopyu.com/ Nikon MicroscopyU] | *[http://www.microscopyu.com/ Nikon MicroscopyU] | ||
Latest revision as of 11:13, 7 March 2013
General Information
- Guidelines for New Members
- Lab Duties
- Standard Operating Procedures for the Equipment
- Standard Operating Procedures for the Protocol
- Common Area Etiquette
- Locations of Common Items and Reagents
- Enzymes
- Abbreviations
- Equipment
- Computing
Protocols
- Media
- Buffers
- Culturing Yeast
- Non-selective Mating of Yeast
- Sporulation in Liquid Media
- PCR
- Kinase and Anneal Oligos
- Restriction Digestion
- Agarose gel electrophoresis
- Gel Extraction
- DNA Ligation (OpenWetWare)
- Making Chemically Competent Bacteria
- Making Electro-competent Bacteria
- Bacterial Transformation
- Yeast Transformation
- Bacterial Plasmid Miniprep
- Yeast Transformation by Electroporation
- Yeast Genomic Prep
- Yeast Plasmid Prep
- Ethanol Precipitation of Nucleic Acids
- Yeast Protein Prep
- SDS-PAGE
- Western Blotting
- mRNA FISH
- Micrococcal Nuclease Assay
- RT-PCR
- Fluorescence Microscopy
- Cromatin Conformation Capture (3C)
- Microfluidic Experiments
Software
- MATLAB
- VectorNTI; speak to the Computer Guru about obtaining a copy. VectorNTI tutorials & reference guide
- Cell tracking and analysis for time-lapse fluorescent microscopy
