Maheshri:Locations: Difference between revisions
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==Descriptions of Locations== | ==Descriptions of Locations== | ||
Shelf | Shelf the shelves located above the media making station | ||
Cupboard | Cupboard the large glass cupboards facing the media making station | ||
Side Shelf | Side Shelf the shelves underneath the windows | ||
Chemical Hood 1 underneath the chemical hood to the right of the main entrance | Chemical Hood 1 underneath the chemical hood to the right of the main entrance | ||
Chemical Hood 2 underneath the chemical hood to the left of the main entrance | Chemical Hood 2 underneath the chemical hood to the left of the main entrance | ||
Drawer the drawers underneath the media making station | Drawer the drawers underneath the media making station | ||
Large Fridge (4C) | Large Fridge (4C) the large fridge between the large cupboards and secondary entrance | ||
Small Fridge (4C) | Small Fridge (4C) the small fridge underneath iGEM's bench | ||
Large Freezer (-20C) | Large Freezer (-20C) the -20C freezer next to Chemical Hood 1 | ||
Small Freezer (-20C) | Small Freezer (-20C) the small fridge/freezer underneath Narendra's bench | ||
Freezer (-80C) the -80C freezer next to Chemical Hood 2 | Freezer (-80C) the -80C freezer next to Chemical Hood 2 | ||
==Chemicals== | ==Chemicals== | ||
Revision as of 16:10, 15 March 2010
Descriptions of Locations
Shelf the shelves located above the media making station Cupboard the large glass cupboards facing the media making station Side Shelf the shelves underneath the windows Chemical Hood 1 underneath the chemical hood to the right of the main entrance Chemical Hood 2 underneath the chemical hood to the left of the main entrance Drawer the drawers underneath the media making station Large Fridge (4C) the large fridge between the large cupboards and secondary entrance Small Fridge (4C) the small fridge underneath iGEM's bench Large Freezer (-20C) the -20C freezer next to Chemical Hood 1 Small Freezer (-20C) the small fridge/freezer underneath Narendra's bench Freezer (-80C) the -80C freezer next to Chemical Hood 2
Chemicals
Name Location 2-Mercaptoethanol Shelf 2-Phenylethanol ? 20X SSC Buffer Shelf Acetic acid Chemical Hood 2 Acetone Chemical Hood 2 Adenine Shelf Ammonium acetate Shelf Ammonium bicarbonate Shelf Ammonium sulfate Shelf Ampicillin Large Freezer (-20C) Bacto agar Shelf Bacto yeast extract Shelf BSA fraction V, RNase/DNase-free Large Freezer (-20C) Buffer for pH meter Shelf Butyl Alcohol Chemical Hood 2 Calcium chloride dihydrate Shelf CarGille Immersion Oil DF Microscope room drawer Chloramphenicol Shelf CYCLOHEXIMIDE Large Freezer (-20C), Shelf DAPI / 4',6-diamidino-2-phenylindole Large Freezer (-20C) D(+)-Galactose Shelf D-Glycogen ? Dextran sulfate Shelf Dextrose Shelf Dimethylsulfoxide Shelf Dithiothreitol Small Fridge (4C) Doxycycline Small Fridge (4C) Ethidium bromide Small Fridge (4C) Ethyl alcohol Chemical Hood 2 Ethylenedinitrotetraacetic acid disodium salt ? Formaldehyde 37% in water Chemical Hood 2 Formamide Chemical Hood 2 Gentamycin sulfate Shelf Glycerin Shelf Glycine Shelf Hepes free acid Shelf Hydrochloric acid Chemical Hood 1 IPTG Large Freezer (-20C) Isopropyl Alcohol Chemical Hood 2 L-(+)-ArGinine Monohydrochloride Shelf L-(+)-Histidine Shelf L-Aspartic acid Shelf L-Glutamic acid monosodium salt monohydrate Shelf L-Isoleucine Shelf L-Leucine Shelf L-Lysine Monohydrate Shelf L-Methionine Shelf L-Phenylalanine Shelf L-Threonine Shelf L-Tryptophan Shelf L-Tyrosine disodium salt, dihydrate Shelf L-Valine Shelf Lactic acid, 85% in water Chemical Hood 2 Lithium acetate Shelf Lithium chloride Shelf Low Melting Agarose Shelf Magnesium Acetate, tetrahydrate Shelf Magnesium Chloride Shelf Manganese Chloride, Tetrahydrate Shelf methanol Chemical Hood 2 MOPS Shelf N,N-Dimethylformamide Chemical Hood 2 NITRIC ACID Chemical Hood 1 NP 40 Shelf NPROTEIN A SEPHAROSE 4 FAST FLOW ? Peptone Shelf Petroleum ether Chemical Hood 2 Phenol/Tris buffered solution Small Fridge (4C) Phenolchloroform Small Fridge (4C) Phosphoric acid Chemical Hood 1 Piperazine1,4bis(2-ethanesulfonic acid) Shelf PMSF ? Poly-L-lysine Hydrobromide ? Polyethylene Glycol 3350 Shelf Potassium Acetate Shelf Potassium Chloride Shelf Raffinose, 5-Hydrate Shelf Ribonucleic acid (RNA) ? Rubidium chloride Shelf Sodium Azide Shelf Sodium Bicarbonate Shelf Sodium Chloride Shelf Sodium deoxycholate ? Sodium Dodecyl Sulfate Shelf Sodium Hydroxide Shelf Sorbitol Shelf SYBR Green I nucleic acid gel stain in DMSO Large Freezer (-20C) Tris Shelf Tris-HCl Shelf Triton Shelf Tryptone Shelf Tryptophol Shelf Tween 20 Shelf Uracil Shelf Urea Shelf Vanadyl Ribonucleoside ? Water Shelf X-Gal Large Freezer (-20C) YNB without amino acids Shelf Bradford reagent Small Fridge (4C) Orange G Small Fridge (4C) Lyticase Small Fridge (4C) L-Canavanine Sulfate Small Fridge (4C) Phenol:Chloroform Small Fridge (4C) FA lysis buffer Small Fridge (4C) Sol I (Miniprep) Small Fridge (4C) Sol III (Miniprep) Small Fridge (4C) Proteinase K Large Freezer (-20C) CelLytic Large Freezer (-20C) Protease Inhibitor Cocktail Large Freezer (-20C) Ligase Large Freezer (-20C) Antarctic phosphatase Large Freezer (-20C) DNAse I Large Freezer (-20C) M-MulV reverse transcriptase Large Freezer (-20C) DNA ladder Large Freezer (-20C) Protein ladder Large Freezer (-20C) T4 polynucleotide kinase Large Freezer (-20C) RNAse A Large Freezer (-20C) Yeast extract Shelf
Lab Supplies
Name Location Microtube (1.5mL) Cupboard Tips (1mL) Cupboard Tips (300uL) Cupboard Tips (10uL) Cupboard Graduated cylinder Cupboard Beaker Cupboard Flask (25mL, 50mL, 2L)) Cupboard Flask (2.8L, wide for cell culture) Cupboard YPD plate Cupboard SD dropout plate Cupboard SPO plate Cupboard Test tube (Small) Cupboard Test tube (Large) Cupboard Test tube cap Drawer Weighing paper Drawer Weighing dishes Drawer Serological pipet (25mL) Drawer Serological pipet (10mL) Drawer Serological pipet (5mL) Drawer Petri dishes Bradley bench's drawer Glass beads, acid-washed Narendra bench's drawer MF75 Vacuum Filter Units Cupboard Falcon tube (50mL) Cupboard Falcon tube (15mL) Cupboard Syringe Drawer Syringe filter (0.45um, 0.2um) Cupboard PCR tube Cupboard Racks Side Shelf Tips (Autoclaved) Side Shelf Toothpick (Autoclaved) Side Shelf Sticks (Autoclaved) Side Shelf Deep well plate (Autoclave) Side Shelf Reservoir of multi-channel pipettes Cupboard Gloves Cupboard Amino acid solution (dropout) Large Fridge (4C) TB amp plate Large Fridge (4C) LB plate Large Fridge (4C) 5' FOA plate Large Fridge (4C) YPD G418 plate Large Fridge (4C) Bottles Cupboard 96 well plate Sink next to TL's bench SDS PAGE equipment Shelf next to the whiteboard Western blot equipment Shelf next to the whiteboard Tool box Side Shelf semi-micro cuvettes Side Shelf Aluminum wrap Shelf next to the whiteboard Plastic wrap Shelf next to the whiteboard
Stocks and Common Reagents
Name Location Bacteria stock Freezer (-80C) Yeast stock Freezer (-80C) Vector stock Large Freezer (-20C) dNTP mix Large Freezer (-20C) ssDNA Large Freezer (-20C) Ampicillin stock Large Freezer (-20C) Restriction Enzyme Large Freezer (-20C) Restriction Enzyme Buffer Large Freezer (-20C) Poly lysine Large Freezer (-20C) TOP 10 competent cell Freezer (-80C) K12 competent cell Freezer (-80C)
