Maheshri:Media: Difference between revisions
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For 1L: | For 1L: | ||
20g Peptone | 20g Peptone | ||
10g Yeast Extract | 10g Yeast Extract | ||
40mL 50% Glucose | |||
=2xYPAD= | =2xYPAD= | ||
For 1L: | |||
20g Yeast Extract | |||
40g Peptone | |||
80mL 50% Glucose | |||
200mg Adenine | |||
=Amino Acid Solution, Complete= | =Amino Acid Solution, Complete= | ||
Revision as of 11:21, 5 September 2009
Yeast Media
YePD/YPD
For 1L:
20g Peptone
10g Yeast Extract
40mL 50% Glucose
2xYPAD
For 1L:
20g Yeast Extract
40g Peptone
80mL 50% Glucose
200mg Adenine
Amino Acid Solution, Complete
For 1L of 10x stock (good for 10L of media)
Adenine 0.4g Arginine 0.2g Histidine 0.2g Isoleucine 0.3g Leucine 1.0g Lysine 0.3g Methionine 0.2g Phenylalanine 0.5g Threonine 2.0g Tryptophan 0.4g Tyrosine 0.3g Uracil 0.2g Valine 1.5g
Bacterial Media
LB (Luria Broth)
For 1L:
10g Trypton
5g Yeast Extract
10g NaCl
TB (Terrific Broth)
For 1L:
10g Trypton
20g Yeast Extract
4mL Glycerol
