Maheshri:Media

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Yeast Media

YePD/YPD

For 1L:

Peptone 20g

Yeast Extract 10g

50% Glucose 40mL

YPAD,2X

For 1L:

Yeast Extract 20g

Peptone 40g

50% Glucose 80mL

Adenine 200mg

SD min

For 1L:

Yeast Nitrogen Base w/o Amino Acids 6.7g

50% Glucose 40mL

Amino Acid Solution, Complete

For 1L of 10x stock (good for 10L of media)

Adenine          0.4g
Arginine         0.2g
Histidine        0.2g
Isoleucine       0.3g
Leucine          1.0g
Lysine           0.3g
Methionine       0.2g
Phenylalanine    0.5g
Threonine        2.0g
Tryptophan       0.4g
Tyrosine         0.3g
Uracil           0.2g
Valine           1.5g

Bacterial Media

LB (Luria Broth)

For 1L:

Trypton 10g

Yeast Extract 5g

NaCl 10g

TB (Terrific Broth)

For 1L:

Trypton 10g

Yeast Extract 20g

Glycerol 4mL