Maheshri:ProteinPrepY: Difference between revisions
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(New page: # Grow 3-5 ml of yeast culture for each sample # Collect sample into Eppendorf tubes, freeze with liquid nitrogen to stop protein expression # To prepare cell lysates, boil the sample for...) |
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Revision as of 12:54, 30 March 2010
- Grow 3-5 ml of yeast culture for each sample
- Collect sample into Eppendorf tubes, freeze with liquid nitrogen to stop protein expression
- To prepare cell lysates, boil the sample for 5 minutes in hot SDS-PAGE sample buffer (50 mM Tris-HCl pH 7.5, 5% SDS, 5% glycerol, 50 mM DDT, 5mM EDTA, complete protease inhibitors). Spin
Total protein content in the lysate was quantified by Bradford assay reagent (Bio-rad).
