Making RNA probes with T7 transcription: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(mRNA sense, probe antisense) |
(In vitro T7 transcription of an RNA probe) |
||
| Line 1: | Line 1: | ||
== mRNA sense & antisense probes == | == mRNA sense & antisense probes == | ||
[[Image:Antisense_probes_and_mRNA.png|thumb|left|500px|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]] | [[Image:Antisense_probes_and_mRNA.png|thumb|left|500px|Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.]] | ||
== In vitro T7 transcription of an RNA probe == | |||
[[Image:T7 primer design for RNA antisense probes.png|thumb|left|500px|Designing primers for T7 RNA synthesis]] | |||
# designing a suitable primer combining the T7 promoter sequence and a sequence specific part | |||
# attaching a T7 promoter via PCR | |||
# in vitro transcription using T7 polymerase and the PCR product as a template | |||
== See also == | == See also == | ||
Revision as of 08:27, 20 June 2009
mRNA sense & antisense probes
In vitro T7 transcription of an RNA probe
- designing a suitable primer combining the T7 promoter sequence and a sequence specific part
- attaching a T7 promoter via PCR
- in vitro transcription using T7 polymerase and the PCR product as a template
