Making RNA probes with T7 transcription

mRNA sense & antisense probes

Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.

See also

• In vitro transcription with T7 RNA polymerase
• Primer design

External links

• T7 transcription protocol by Jonathan Davies of Harvard Uni (1998)
• In vitro transcription basics at Ambion
• T7 RNA polymerase - Wikipedia, RNA polymerases - Fermentas]