Making RNA probes with T7 transcription

From OpenWetWare

Revision as of 11:33, 20 June 2009 by Jakob Suckale (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
back to protocols

Contents

mRNA sense & antisense probes

Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.
Probes need to be antisense respective to the mRNA to be able to bind their target. Care has to be taken during experimental design to make a suitable antisense probe.


In vitro T7 transcription of an RNA probe

Designing primers for T7 RNA synthesis
Designing primers for T7 RNA synthesis


  1. designing a suitable primer combining the T7 promoter sequence and a sequence specific part
  2. attaching a T7 promoter via PCR
  3. in vitro transcription using T7 polymerase and the PCR product as a template


See also

External links

Personal tools