Malaria

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Current revision (19:46, 1 February 2012) (view source)
(OpenWetWare Projects on Malaria)
 
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== OpenWetWare Projects on Malaria ==
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A list of OpenWetWare projects on Malaria:
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*[[Open_Source_Drug_Discovery_-_Malaria|Open Source Drug Discovery - Malaria]]
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*[[Crisanti:Projects|Crisanti Group]]
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== Live replicating adenovirus malaria vaccine ==
== Live replicating adenovirus malaria vaccine ==
== Plasmids ==
== Plasmids ==
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*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
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*Progenitor - [[pTG3602]] - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
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**pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid [[Media:pCP08.xdna]]
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**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
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**pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
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**[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
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**pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
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**[[pCP03]] - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
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all plasmids are AmpR
all plasmids are AmpR
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== Viruses ==
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*Ad5 full genome capsid display mutants
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**CS (circumsporozoite antigent) mutants
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***Gk2 - HVR1 (NANP)5 subsitution
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***GKIII-2 - HVR5 T* insertion between hexon AAs 272 and 273
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*Ad5 late gene expression mutants
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**LSA3 (liver stage antigen 3)
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***FFH3 - hexon splice aceptor

Current revision

Contents

OpenWetWare Projects on Malaria

A list of OpenWetWare projects on Malaria:

Live replicating adenovirus malaria vaccine

Plasmids

  • Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
    • pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
    • pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
    • pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid

all plasmids are AmpR


Viruses

  • Ad5 full genome capsid display mutants
    • CS (circumsporozoite antigent) mutants
      • Gk2 - HVR1 (NANP)5 subsitution
      • GKIII-2 - HVR5 T* insertion between hexon AAs 272 and 273
  • Ad5 late gene expression mutants
    • LSA3 (liver stage antigen 3)
      • FFH3 - hexon splice aceptor
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