Malaria: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 4: | Line 4: | ||
== Plasmids == | == Plasmids == | ||
*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA | *Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA | ||
**pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | **pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid [[file:pCP08.xdna]] | ||
**pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid | **pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid | ||
**pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid | **pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid | ||
all plasmids are AmpR | all plasmids are AmpR |
Revision as of 08:24, 30 August 2006
Live replicating adenovirus malaria vaccine
Plasmids
- Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
- pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid File:PCP08.xdna
- pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
- pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
all plasmids are AmpR