Malaria
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(Difference between revisions)
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== Plasmids == | == Plasmids == | ||
| - | *Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA | + | *Progenitor - [[pTG3602]] - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA |
**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | **[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | ||
| - | ** | + | **[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid |
| - | + | ||
all plasmids are AmpR | all plasmids are AmpR | ||
| + | **[[pCP03]] - Ad5 with NdeI site in fiber removed - hexon recombination plasmid | ||
Revision as of 11:31, 30 August 2006
Live replicating adenovirus malaria vaccine
Plasmids
- Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
all plasmids are AmpR
- pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
