Malaria

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== Plasmids ==
== Plasmids ==
-
*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
+
*Progenitor - [[pTG3602]] - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
-
**pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
+
**[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
-
**pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
+
all plasmids are AmpR
all plasmids are AmpR
 +
**[[pCP03]] - Ad5 with NdeI site in fiber removed - hexon recombination plasmid

Revision as of 10:31, 30 August 2006

Live replicating adenovirus malaria vaccine

Plasmids

  • Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
    • pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
    • pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid

all plasmids are AmpR

    • pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid
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