Malaria: Difference between revisions
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== Plasmids == | == Plasmids == | ||
*Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA | *Progenitor - [[pTG3602]] - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA | ||
**[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | **[[pCP08]] - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid | ||
** | **[[pCP05]] - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid | ||
all plasmids are AmpR | all plasmids are AmpR | ||
**[[pCP03]] - Ad5 with NdeI site in fiber removed - hexon recombination plasmid |