Malaria

(Difference between revisions)

Revision as of 11:39, 30 August 2006

Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
- pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
- pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
- pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid

all plasmids are AmpR

Ad5 full genome capsid display mutants
- CS (circumsporozoite antigent) mutants
  - Gk2 - HVR1 (NANP)5 subsitution
  - GKIII-2 - HVR5 T* insertion between hexon AAs 272 and 273

@@ Line 9: / Line 9: @@
 all plasmids are AmpR
+== Viruses ==
+*Ad5 full genome capsid display mutants
+**CS (circumsporozoite antigent) mutants
+***Gk2 - HVR1 (NANP)5 subsitution
+***GKIII-2 - HVR5 T* insertion between hexon AAs 272 and 273