Malaria

Plasmids

• Progenitor - pTG3602 - whole Ad5 genome with pPolyII linking ITRs. Cut with PacI to liberate infectious Ad5 DNA
  • pCP08 - Ad5 with NdeI site in fiber removed and lacZ from pUC19 stuck into NdeI site in hexon - hexon recombination plasmid
  • pCP05 - Ad5 with hexon deletion (15671bp-25290bp) - fiber recombination plasmid
  • pCP03 - Ad5 with NdeI site in fiber removed - hexon recombination plasmid

all plasmids are AmpR

Viruses

• Ad5 full genome capsid display mutants
  • CS (circumsporozoite antigent) mutants
    • Gk2 - HVR1 (NANP)5 subsitution
    • GKIII-2 - HVR5 T* insertion between hexon AAs 272 and 273