Maloof Lab:96well EtOH precipitation

From OpenWetWare
Revision as of 14:07, 4 June 2008 by Jmjimenez (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home      Research      Publications      Protocols      Resources      Announcements      Lab Safety     

96-Well Format DNA EtOH Precipitation with glycogen

  1. Add 1/10 the volume of your sample of 3M sodium acetate
  2. Add 1 uL glycogen per mL of above solution
  3. Add 2.5x total volume of 100% cold ethanol
  4. Incubate at -20 C for 15 minutes
  5. Spin at 4 C at top speed for 15 minutes
  6. Remove supernatant
  7. Allow pellet to dry
  8. Resuspend in TE
Retrieved from "https://openwetware.org/mediawiki/index.php?title=Maloof_Lab:96well_EtOH_precipitation&oldid=209473"