Maloof Lab:Hypocotyl measurement

From OpenWetWare

Revision as of 21:42, 5 January 2009 by Jmjimenez (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Image:logo2_final.jpg

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home      Research      Publications      Protocols      Resources      Announcements      Lab Safety     

Hypocotyl measurement with ImageJ



Needed

  1. ImageJ (from http://rsb.info.nih.gov/ij/)
  2. Working scanner (can be hard)

Scanner Settings

  1. Set to Grayscale.
  2. Set to 600 ppi.
  3. Set to wtb points.
  4. Set to Custom ~130,255.
  5. Set Preferences -> Scan Material -> Positive.
  6. Set Preferences -> More -> Scan Quality -> Quality.
  7. Set Preferences -> More -> Interpolation -> Speed.

Image J

  1. Go to Analyze -> Set Scale.
  2. Over there change:

Distance in Pixels -> 600

Known Distance -> 25.4

Pixel Aspect Ratio -> 1

Unit of Length -> mm

Personal tools