MS plates for plant growth
This protocol will make 4 liters. Adjust to your needs if necessary.
- Put 3500 ml Milli-Q H2O in a beaker
- Weight 17.6 g of MSMO (in the 4°C freezer, 4.4 per liter) and add it to the water (agitating)
- Add 30 g/l (120 g for 4 liters) Sucrose
- Adjust pH to 5.7 (adding KOH if it’s lower)
- Fill with Milli-Q water to exactly 4 liters
- Put 7 g of Agar in 4 clean glass bottles and add 1 liter of the mix to each bottle
- Autoclave for 35 min
Let cool, add antobiotic to you rconvenience and pour into plates
Big round plates take about 50 ml of media
Square plates take about 25 ml of media
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