Maloof Lab:MS plates

MS plates for plant growth

This protocol will make 4 liters. Adjust to your needs if necessary.

1. Put 3500 ml Milli-Q H₂O in a beaker
2. Weight 17.6 g of MSMO (in the 4°C freezer, 4.4 per liter) and add it to the water (agitating)
3. Add 30 g/l (120 g for 4 liters) Sucrose
4. Adjust pH to 5.7 (adding KOH if it’s lower)
5. Fill with Milli-Q water to exactly 4 liters
6. Put 7 g of Agar (per bottle) in 4 clean glass bottles and add 1 liter of the mix to each bottle
7. Autoclave for 35 min

Let cool, add antibiotic to your convenience and pour into plates
Big round plates take about 50 ml of media
Square plates take about 25 ml of media