Maloof Lab:MS plates2: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 11: | Line 11: | ||
#Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar. | #Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar. | ||
#Put 700 ml Milli-Q H<sub>2</sub>O in a beaker | #Put 700 ml Milli-Q H<sub>2</sub>O in a beaker | ||
#Weight | #Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating) | ||
#Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid) | #Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid) | ||
#Adjust pH to 5.8 (adding KOH) | #Adjust pH to 5.8 (adding KOH) |
Revision as of 15:52, 25 October 2012
Room 2115 |
MS plates for plant growth (buffered, no sucrose)This protocol will make 1 liters. Adjust to your needs if necessary.
created by Kazu (Oct 25, 2012) |