Maloof Lab:MS plates2

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 11: Line 11:
#Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar.
#Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar.
#Put 700 ml Milli-Q H<sub>2</sub>O in a beaker
#Put 700 ml Milli-Q H<sub>2</sub>O in a beaker
-
#Weight 4.4 g of MSMO (in the 4°C freezer) and add it to the water (agitating)
+
#Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating)
#Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid)  
#Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid)  
#Adjust pH to 5.8 (adding KOH)
#Adjust pH to 5.8 (adding KOH)

Revision as of 18:52, 25 October 2012

Image:logo2_final.jpg

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home      Research      Publications      Protocols      Resources      Announcements      Lab Safety     

MS plates for plant growth (buffered, no sucrose)


This protocol will make 1 liters. Adjust to your needs if necessary.

  1. Final concentration of this medium is 1/2 MSMO, 5mM MES (pH5.8), 0.8% (or 0.7%) agar.
  2. Put 700 ml Milli-Q H2O in a beaker
  3. Weight 2.2 g of MSMO (in the 4°C freezer) and add it to the water (agitating)
  4. Add 1.0g of MES (2-(N-morpholino)ethanesulfonic acid)
  5. Adjust pH to 5.8 (adding KOH)
  6. Fill with Milli-Q water to exactly 1 liters
  7. Put 8 g (for vertical plate) or 7 g (for horizontal plate) of Agar in 1 clean glass bottle
  8. Autoclave for 20 min


Let cool, add antibiotic to your convenience and pour into plates
Big round plates take about 50 ml of media
Square plates take 25 ml of media (for horizontal plate) or 40 ml of media (for vertical plate).

created by Kazu (Oct 25, 2012)
Personal tools