Maloof Lab:PCR stuff: Difference between revisions
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<h3><font style="color:#F8B603;">Taq polymerase</font></h3> | <h3><font style="color:#F8B603;">Taq polymerase</font></h3> | ||
:1 (current ('07) stock Taq) : | :1 (current ('07) stock Taq) : 9 (storage buffer) | ||
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Revision as of 10:37, 28 January 2008
Room 2115 |
Important: Label tubes with buffer name, date, your initials, and tube number 10x PCR buffer (see Promega PCR buffer) for 20 ml
Add sterile MilliQ up to 20 ml in total. Note 1: Renew 10xPCR buffer every 1~2 months. Taq polymerase
MgCl2 (25 mM)
300 µl aliquots in 0.5 ml tubes. dNTP (100mM total, 25 mM each)
Make 100 µl aliquots. |