Maloof Lab:Protocols

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[[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]]
[[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]]
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[[Maloof_Lab:Cell_Transformation|<font style="color:#000;">Cell transformation</font>]]
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[[Maloof_Lab:Cell_Transformation|<font style="color:#000;">E. coli (DH5α chemiocompetent) transformation</font>]]
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[[Maloof_Lab:Agro_Transformation|<font style="color:#000;">Agrobacterium transformation using the Freeze-Thaw method</font>]]
[[Maloof_Lab:Cleaning_Ball_Bearings|<font style="color:#000;">Cleaning ball bearings</font>]]
[[Maloof_Lab:Cleaning_Ball_Bearings|<font style="color:#000;">Cleaning ball bearings</font>]]

Revision as of 14:44, 10 July 2008

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Protocols

DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

E. coli (DH5α chemiocompetent) transformation

Agrobacterium transformation using the Freeze-Thaw method

Cleaning ball bearings

Seed sterilization

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Exo-SAP PCR cleanup for sequencing

Stocks

PCR taq, buffer and MgCl2

Antibiotics

Electrophoresis Buffers

Loading Dye

Lab Safety

Maloof lab safety

Scripts, instructions and miscellaneous

Using the LI1800 light meter

Analyzing luminiscence over time with ImageJ


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