Maloof Lab:Protocols

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[[Maloof_Lab:Seed_Storage|Seed Storage]]
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[http://www.plantcell.org/cgi/content/full/20/7/1736 Eleven Golden Rules for q-RT-PCR (Uvardi et al.)]
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[http://www.plantcell.org/cgi/content/full/20/7/1736 Eleven Golden Rules of qRT-PCR (Uvardi et al.)]
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Revision as of 14:44, 6 September 2008

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Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

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Protocols

DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

E. coli (DH5α chemically-competent) transformation

Agrobacterium transformation (Freeze-Thaw method)

Cleaning ball bearings

Seed sterilization

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Exo-SAP PCR cleanup for sequencing

Seed Storage

Eleven Golden Rules of qRT-PCR (Uvardi et al.)

Stocks

PCR taq, buffer and MgCl2

Antibiotics

Electrophoresis Buffers

Loading Dye

Lab Safety

Maloof lab safety

Lab Tasks

Maloof lab tasks

Scripts, instructions and miscellaneous

Using the LI1800 light meter

Analyzing luminiscence over time with ImageJ


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