Maloof Lab:Protocols: Difference between revisions
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[[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]] | [[Maloof_Lab:96well_EtOH_precipitation|<font style="color:#000;">DNA precipitation for 96 well plates, with glycogen</font>]] | ||
[[Maloof_Lab: | [[Maloof_Lab:Ultra-competent E.coli|<font style="color:#000;">Making ultra-competent E.coli (Inoue method)</font>]] | ||
[[Maloof_Lab:Agro_Transformation|<font style="color:#000;">Agrobacterium transformation | [[Maloof_Lab:Cell_Transformation|<font style="color:#000;">E. coli (DH5α chemically-competent) transformation</font>]] | ||
[[Maloof_Lab:Agro_Transformation|<font style="color:#000;">Agrobacterium transformation (Freeze-Thaw method)</font>]] | |||
[[Maloof_Lab:Cleaning_Ball_Bearings|<font style="color:#000;">Cleaning ball bearings</font>]] | [[Maloof_Lab:Cleaning_Ball_Bearings|<font style="color:#000;">Cleaning ball bearings</font>]] | ||
[[Maloof_Lab:Seed_Sterilization|<font style="color:#000;"> | [[Maloof_Lab:Seed_Sterilization|<font style="color:#000;">Arabidopsis seed sterilization</font>]] | ||
[[Maloof_Lab:miniprep_w_promega_reagents|<font style="color:#000;">Miniprep using Promega reagents but not the columns</font>]] | [[Maloof_Lab:miniprep_w_promega_reagents|<font style="color:#000;">Miniprep using Promega reagents but not the columns</font>]] | ||
[[Maloof_Lab:miniprep_reagents|<font style="color:#000;">Recipes for Miniprep solutions</font>]] | |||
[[Maloof_Lab:MS_plates|<font style="color:#000;">MS plates for plant growth</font>]] | [[Maloof_Lab:MS_plates|<font style="color:#000;">MS plates for plant growth</font>]] | ||
[[Maloof_Lab:MS_plates2|<font style="color:#000;">MS plates for plant growth (buffered, no sucrose)</font>]] | |||
[[Maloof_Lab:Exo_SAP|<font style="color:#000;">Exo-SAP PCR cleanup for sequencing</font>]] | [[Maloof_Lab:Exo_SAP|<font style="color:#000;">Exo-SAP PCR cleanup for sequencing</font>]] | ||
[[Maloof_Lab:Seed_Storage|<font style="color:#000;">Seed Storage</font>]] | |||
[http://www.plantcell.org/cgi/reprint/20/7/1736 <font style="color:#000;">Eleven Golden Rules of qRT-PCR (Uvardi et al.)</font>] | |||
[[Maloof_Lab:Trizol|<font style="color:#000;">Trizol extraction</font>]] | |||
[[Maloof_Lab:Tomato seed TMV treatment and sterilization|<font style="color:#000;">Tomato seed TMV treatment and sterilization</font>]] | |||
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<h3><font style="color:#C9D3EB;">Stocks</font></h3> | <h3><font style="color:#C9D3EB;">Stocks</font></h3> | ||
[[Maloof_Lab:antibiotics|<font style="color:#000;">Antibiotics</font>]] | [[Maloof_Lab:antibiotics|<font style="color:#000;">Antibiotics</font>]] | ||
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[[Maloof_Lab:buffers_electrophoresis|<font style="color:#000;">Electrophoresis Buffers</font>]] | [[Maloof_Lab:buffers_electrophoresis|<font style="color:#000;">Electrophoresis Buffers</font>]] | ||
[[Maloof_Lab:loading_dye|<font style="color:#000;">Loading Dye</font>]] | [[Maloof_Lab:loading_dye|<font style="color:#000;">Loading Dye and 1kb Ladder</font>]] | ||
[[Maloof_Lab:PCR_stuff|<font style="color:#000;">PCR taq, buffer and MgCl<sub>2</sub></font>]] | |||
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<h3><font style="color:red'">Lab Safety</font></h3> | <h3><font style="color:red'">Lab Safety</font></h3> | ||
[[Maloof_Lab:safety|<font style="color:#000;">Maloof lab | [[Maloof_Lab:Lab_Safety|<font style="color:#000;">Harmer/Maloof labs' safety page</font>]] | ||
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<h3><font style="color:lightblue;">Lab Tasks</font></h3> | |||
[[Maloof_Lab:Lab_Tasks|<font style="color:#000;">Maloof lab tasks</font>]] | |||
[[Maloof_Lab:Undergrad_Instructions|<font style="color:#000;">Undergrad Instructions</font>]] | |||
|width=550px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" | | |width=550px style="padding: 5px; background-color: #ffffff; border: 2px solid #F8B603;" | | ||
<h3><font style="color:#F8B603;">Scripts, instructions and miscellaneous</font></h3> | <h3><font style="color:#F8B603;">Scripts, instructions and miscellaneous</font></h3> | ||
[[Maloof_Lab:Epson_Scanner|<font style="color:#000;">Using the Epson scanner</font>]] | |||
[[Maloof_Lab:LI1800_light_meter|<font style="color:#000;">Using the LI1800 light meter</font>]] | [[Maloof_Lab:LI1800_light_meter|<font style="color:#000;">Using the LI1800 light meter</font>]] | ||
[[Media:Black_Comet_V1.doc|<font style="color:#000;">Using the Black Comet light meter</font>]] | |||
[[Maloof_Lab:eBioX|<font style="color:#000;">Sequence Analysis with eBioX</font>]] | |||
[[Maloof_Lab:ImageJ_analysis|<font style="color:#000;">Analyzing luminiscence over time with ImageJ</font>]] | [[Maloof_Lab:ImageJ_analysis|<font style="color:#000;">Analyzing luminiscence over time with ImageJ</font>]] | ||
[[Maloof_Lab:Hypocotyl_measurement|<font style="color:#000;">Hypocotyl measurement with ImageJ</font>]] | |||
[http://ostrander.ucdavis.edu/~maloofweb/cgi-bin/ <font style="color:#000;">Maloof/Harmer Primer Database Search</font>] (Only internal users) | |||
[http://ostrander.ucdavis.edu/~maloofweb/cgi-bin/Patricia <font style="color:#000;">Patricia's Motif search </font>] (Only internal users) | |||
[http://whitney.plb.ucdavis.edu/gene_exp_plot/form.html <font style="color:#000;">Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN</font>] (Only internal users) | |||
[http://whitney.plb.ucdavis.edu/gene_exp_4_species/form.html <font style="color:#000;">Maloof Gene Expression Plot Tool for M82, PEN, HAB & PIM</font>] (Only internal users) | |||
[[Maloof_Lab:Laser_tough_tags_settings|<font style="color:#000;">Laser Tough Tag Settings to print</font>]] | |||
[[Maloof_Lab:How_to_print_barcodes|<font style="color:#000;">How to print barcodes</font>]] | |||
[[Maloof_Lab:PDA_bluetooth_calipers|<font style="color:#000;">PDA measurement system</font>]] | |||
[[Maloof_Lab:ImageJ_Time_Lapse_Hypocotyls|<font style="color:#000;">Measuring time-lapse hypocotyl movies with ImageJ</font>]] | |||
[[Maloof_Lab:Instructions for champion for the earth|<font style="color:#000;">Earth champion instructions</font>]] | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 14:50, 21 October 2014
Room 2115 |
Lab SafetyHarmer/Maloof labs' safety page
|
Lab Tasks |
Scripts, instructions and miscellaneousUsing the Black Comet light meter Analyzing luminiscence over time with ImageJ Hypocotyl measurement with ImageJ Maloof/Harmer Primer Database Search (Only internal users) Patricia's Motif search (Only internal users) Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN (Only internal users) Maloof Gene Expression Plot Tool for M82, PEN, HAB & PIM (Only internal users) Laser Tough Tag Settings to print |