Maloof Lab:Protocols

Room 2115 Section of Plant Biology 1002 Life Sciences, One Shields Ave. University of California Davis Davis, CA 95616 Contact

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Protocols DNA extraction for 96 well plates, CTAB method DNA precipitation for 96 well plates, with glycogen Making ultra-competent E.coli (Inoue method) E. coli (DH5α chemically-competent) transformation Agrobacterium transformation (Freeze-Thaw method) Cleaning ball bearings Seed sterilization Miniprep using Promega reagents but not the columns MS plates for plant growth Exo-SAP PCR cleanup for sequencing Seed Storage Eleven Golden Rules of qRT-PCR (Uvardi et al.) Trizol extraction

Stocks Antibiotics Electrophoresis Buffers Loading Dye and 1kb Ladder PCR taq, buffer and MgCl2

Lab Safety Maloof lab safety

Lab Tasks Maloof lab tasks Undergrad Instructions

Scripts, instructions and miscellaneous Using the Epson scanner Using the LI1800 light meter Using the Black Comet light meter Sequence Analysis with eBioX Analyzing luminiscence over time with ImageJ Hypocotyl measurement with ImageJ Maloof/Harmer Primer Database Search (Only internal users) Patricia's Motif search (Only internal users) Maloof Gene Expression Plot Tool in different tissues for M82 vs PEN (Only internal users) Maloof Gene Expression Plot Tool for M82, PEN, HAB & PIM (Only internal users) Laser Tough Tag Settings to print PDA measurement system Measuring time-lapse hypocotyl movies with ImageJ