Maloof Lab:Protocols

From OpenWetWare

Revision as of 15:44, 6 September 2008 by Jnmaloof (Talk | contribs)

(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)

Jump to: navigation, search

Room 2115
Section of Plant Biology
1002 Life Sciences, One Shields Ave.
University of California Davis
Davis, CA 95616

Contact

Home Research Publications Protocols Resources Announcements

Protocols

DNA extraction for 96 well plates, CTAB method

DNA precipitation for 96 well plates, with glycogen

E. coli (DH5α chemically-competent) transformation

Agrobacterium transformation (Freeze-Thaw method)

Cleaning ball bearings

Seed sterilization

Miniprep using Promega reagents but not the columns

MS plates for plant growth

Exo-SAP PCR cleanup for sequencing

Eleven Golden Rules for q-RT-PCR (Uvardi et al.)

Stocks

PCR taq, buffer and MgCl₂

Electrophoresis Buffers

Lab Safety

Maloof lab safety

Lab Tasks

Maloof lab tasks

Scripts, instructions and miscellaneous

Using the LI1800 light meter

Analyzing luminiscence over time with ImageJ

Retrieved from "http://www.openwetware.org/wiki/Maloof_Lab:Protocols"

Views

Personal tools

Log in

research

Search

Toolbox

Contributing Authors