Maloof Lab:Protocols

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Protocols DNA extraction for 96 well plates, CTAB method DNA precipitation for 96 well plates, with glycogen E. coli (DH5α chemically-competent) transformation Agrobacterium transformation (Freeze-Thaw method) Cleaning ball bearings Seed sterilization Miniprep using Promega reagents but not the columns MS plates for plant growth Exo-SAP PCR cleanup for sequencing Seed Storage Eleven Golden Rules of qRT-PCR (Uvardi et al.)

Stocks Antibiotics Electrophoresis Buffers Loading Dye PCR taq, buffer and MgCl2

Lab Safety Maloof lab safety

Lab Tasks Maloof lab tasks

Scripts, instructions and miscellaneous Using the LI1800 light meter Analyzing luminiscence over time with ImageJ Maloof/Harmer Primer Database Search (Only internal users) Patricia's Motif search (Only internal users)